Plasmid Collection for Autophagy Research

Plasmids encoding HTT103Q-GFP (1385; from Michael Sherman), FLAG-STX17 (45911; from Noboru Mizushima,) and GFP-STX17 (45909; from Noboru Mizushima,) were obtained from Addgene. Plasmids encoding MYC-BORCS6 and LAMP1-GFP were previously described.²¹ (link) A plasmid encoding GFP-mCherry-LC3B was generated by subcloning a mCherry-LC3B fragment into pEGFP-C1 (Clontech Laboratories, 6084). Plasmids encoding mCherry-VPS41 and mCherry-VPS39 were generated by subcloning the coding sequences from pSX-VPS41 and pSX-VPS39⁴⁹ (link) into pmCherry-C1 (Clontech Laboratories, 632524). A plasmid encoding KXD1-GFP was generated by subcloning a KXD1 fragment from MYC-KXD1²¹ (link) into pEGFP-N1 (Clontech Laboratories, 6085). The LIR-mutated KXD1 (KXD1(ΔLIR)-GFP) was generated by site-directed mutagenesis. The ARL8B-GFP plasmid was a gift from Dr. John Brumell (Hospital for Sick Children, Toronto, Canada). A FLAG-VPS41 plasmid was a gift from Dr. Chihiro Akazawa (Tokyo Medical and Dental University, Tokyo, Japan).