Quantitative RT-PCR Analysis of Mouse Cardiomyocytes
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Corresponding Organization :
Other organizations : Brigham and Women's Hospital, Harvard University, University of Michigan–Ann Arbor, Center for Systems Biology, Massachusetts General Hospital
Variable analysis
- Primer design using Vector NTI Advance 11 (Invitrogen) software
- MRNA expression levels of genes measured by real-time RT-PCR
- Fibrotic markers measured by real-time RT-PCR
- Total RNA extracted from mouse brain and skeletal muscle used as controls
- β-2-microglobulin (β2m) used for normalization of Ct values for gene expression
- Hypoxanthine guanine phosphoribosyl transferase (Hprt) used for normalization of Ct values for fibrotic markers
- Total RNA extracted from mouse brain and skeletal muscle
- No explicit negative controls mentioned
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