Alcohol (95% v/v, Pharmaco-AAPR) was diluted in tap water to 20% v/v. Alcohol was then administered via intragastric gavage (IG) volume-adjusted to achieve a dose of 0.8 g/kg. Sucrose (Great Value brand granulated sugar) was dissolved in tap water to a concentration of 26% w/v. Muscimol and baclofen (R&D systems, Minneapolis, Minnesota) were dissolved in sterile 0.9% saline to produce a cocktail of 0.1 mm Muscimol + 1 mm baclofen, and the doses were chosen based on previous work 18 (link), 31 (link), 32 (link).
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Alcohol, Sucrose, and Neuropharmacological Cocktail Protocol
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Corresponding Organization : University of North Carolina at Chapel Hill
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Variable analysis
independent variables
- Alcohol (95% v/v, Pharmaco-AAPR) diluted in tap water to 20% v/v
- Sucrose (Great Value brand granulated sugar) dissolved in tap water to a concentration of 26% w/v
- Muscimol and baclofen (R&D systems, Minneapolis, Minnesota) dissolved in sterile 0.9% saline to produce a cocktail of 0.1 mm muscimol + 1 mm baclofen
- Unspecified
- Tap water used for dilution and dissolution
- Sterile 0.9% saline used as solvent for muscimol and baclofen
- Doses of muscimol and baclofen chosen based on previous work (references 18, 31, 32)
- No negative controls explicitly mentioned
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