Visualizing D4 Receptors in Rat Aorta

Expression of D₄ receptors in thoracic aorta from SD rats was examined by laser scanning confocal microscope. Briefly, the aorta was cleared of blood with ice-cold oxygenated saline and kept in Histochoice (Amresco, Solon, OH) for one to two days at 4°C, then sectioned (4 μm), embedded in paraffin, and mounted on slides. The sections were double-immunostained with mouse anti-D₄ receptor antibody (1:100) and rabbit polyclonal anti-α-SM-actin antibody (1:100). The colocalization of D₄ receptors and α-SM-actin was performed as described previously [18 (link)].

Free full text: Click here

Yu C., Wang Z., Han Y., Liu Y., Wang W.E., Chen C., Wang H., Jose P.A, & Zeng C. (2014). Dopamine D4 receptors inhibit proliferation and migration of vascular smooth muscle cells induced by insulin via down-regulation of insulin receptor expression. Cardiovascular Diabetology, 13, 97.

Publication 2014

Histochoice

Actin Anti antibody Aorta Blood Cold Confocal laser scanning microscope Embedded paraffin Mouse Rabbit Rats Receptor antibody Saline Solon Thoracic aorta

Corresponding Organization :

Other organizations : Army Medical University, Daping Hospital, University of Maryland, Baltimore

Top 5 similar protocols

Variable analysis

independent variables

Expression of D4 receptors in thoracic aorta

dependent variables

Colocalization of D4 receptors and α-SM-actin

control variables

Tissue preparation (cleared of blood, kept in Histochoice, sectioned, embedded in paraffin, and mounted on slides)
Immunostaining (mouse anti-D4 receptor antibody and rabbit polyclonal anti-α-SM-actin antibody)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!