In this study, 30 patients with clavicle fractures and 3 patients with cholecystectomy were included. All patients were admitted in the Department of Orthopaedic Surgery and Department of Hepatobiliary Surgery of the Third Xiangya Hospital of Central South University from November 2021 to June 2022. All patients were excluded from diabetes, acute infection, tumor, and smoking. The adipose tissue samples were obtained from the supraclavicular fossa of 30 patients with clavicle fractures, but only 3 cases of BAT were confirmed with H&E staining and UCP1 expression. WAT samples were obtained from abdominal subcutaneous tissues of three patients with cholecystectomy surgery; those patients were of same gender, age, and weight with three BAT group patients.
The design of this research work was approved by the Ethics Review Committee of the Third Xiangya Hospital of Central South University, Changsha, China, and the research experiments were performed according to the guidelines outlined in the Declaration of Helsinki. All individual patients provided written informed consent.