Protocol detail

Differentiation of hMSCs into Adipocytes and Osteocytes

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Untransduced hMSCs as well as CEETnl2- and CEETnl2Is2 transduced hMSC were differentiated to adipocytes and osteocytes as previously described76 (link). Briefly, hMSCs were plated in 6-well plates at a density of 20,000 cells/cm² for adipogenesis, 10,000 cells/cm² for osteogenesis and incubated in the adipogenic and osteogenic MSCs differentiation BulletKits respectively (lonza, Basel, Switzerland).

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Benabdellah K., Muñoz P., Cobo M., Gutierrez-Guerrero A., Sánchez-Hernández S., Garcia-Perez A., Anderson P., Carrillo-Gálvez A.B., Toscano M.G, & Martin F. (2016). Lent-On-Plus Lentiviral vectors for conditional expression in human stem cells. Scientific Reports, 6, 37289.

Publication 2016

BulletKits

Adipocytes Adipogenic Cells Osteocytes Osteogenic

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Variable analysis

independent variables

Transduction of hMSCs: untransduced, CEETnl2 transduced, CEETnl2Is2 transduced

dependent variables

Adipocyte differentiation of hMSCs
Osteocyte differentiation of hMSCs

control variables

Cell density for adipogenesis: 20,000 cells/cm^2
Cell density for osteogenesis: 10,000 cells/cm^2
Adipogenic and osteogenic MSCs differentiation BulletKits from Lonza, Basel, Switzerland

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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