Telomere Length SNP Genotyping

Among the 14 SNPs were selected, rs11125529 was chosen from previously published polymorphisms associated with telomere length [13 (link)], others were randomly chosen from the published gene ACYP2 associated with telomere length, with minor allele frequencies >5% in the HapMap Chinese Han Beijing population. We used the GoldMag-Mini Whole Blood Genomic DNA Purification Kit (GoldMag Co. Ltd. Xi’an City, China) extracted from whole blood. Using a NanoDrop 2000 (Gene Company Limited) were measured DNA concentrations. We used Sequenom MassARRAY Assay Design 3.0 Software to design a Multiplexed SNP MassEXTEND assay [19 ]. Sequenom MassARRAY RS1000 was used for genotyping, and the related data were managed using Sequenom Typer 4.0 Software [19 , 20 (link)]. Laboratory personnel were blinded to the genotyping results of all samples.