Genetic Risk Variants in T2DM

DNA was isolated using the Invisorb^® Blood Universal Kit from Stratec Molecular (Berlin, Germany). Eleven well-known T2DM genetic risk variants were genotyped: TCF7L2(rs7903146), PPARG-P12A(rs1801282), DUSP9(rs594532), CENTD2(rs1552224), THADA(rs7578597), HHEX(rs1111875), CDKAL1(rs7754840) and KCNQ1(rs231362)which had previously been genotyped for replication in DIAGRAM [37 (link)], and KCNJ11(rs5219), IGF2BP2(rs4402960) and FTO(rs8050136). These risk variants were chosen because of their relatively large effect sizes on T2DM risk in previous studies [32 (link), 37 (link)–42 (link)]. Genotyping was performed with TaqMan allelic discrimination assays, designed and optimized by Applied Biosystems (Foster City, CA, USA). Reactions were performed on the Taqman Prism 7900 HT platform.

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van Herpt T.T., Lemmers R.F., van Hoek M., Langendonk J.G., Erdtsieck R.J., Bravenboer B., Lucas A., Mulder M.T., Haak H.R., Lieverse A.G, & Sijbrands E.J. (2017). Introduction of the DiaGene study: clinical characteristics, pathophysiology and determinants of vascular complications of type 2 diabetes. Diabetology & Metabolic Syndrome, 9, 47.

Publication 2017

Invisorb® Blood Universal Kit TaqMan allelic discrimination assays

Allelic Assays Blood Cdkal1 Discrimination Genetic variants Igf2bp2 Prism Replication Tcf7l2

Corresponding Organization :

Other organizations : Erasmus MC, Máxima Medisch Centrum, Radboud University Nijmegen, Catharina Ziekenhuis

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Variable analysis

independent variables

TCF7L2(rs7903146)
PPARG-P12A(rs1801282)
DUSP9(rs594532)
CENTD2(rs1552224)
THADA(rs7578597)
HHEX(rs1111875)
CDKAL1(rs7754840)
KCNQ1(rs231362)
KCNJ11(rs5219)
IGF2BP2(rs4402960)
FTO(rs8050136)

dependent variables

Type 2 Diabetes Mellitus (T2DM) risk

control variables

DNA isolation using the Invisorb® Blood Universal Kit from Stratec Molecular
Genotyping performed with TaqMan allelic discrimination assays, designed and optimized by Applied Biosystems
Reactions performed on the Taqman Prism 7900 HT platform

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